Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time

Abstract Background Filling silos generally takes much time in practice, which may negatively affect silage fermentation and feed intake. To clarify the effects of inoculating time, lactic acid bacteria (LAB) strains filling on microbes stylo ( Stylosanthes guianensis ) its silage, ensiling was quickly performed (quickly filling, QF) with or without LAB Lactobacillus plantarum SXC48, Lb. CCZZ1 Enterococcus faecalis XC124), after inoculated placed for 1 day (delayed DF1) 2 days (DF2). Results Delayed significantly increased pH, buffering capacity, microbial counts acid, acetic NH 3 –N contents prior to ensiling. Inoculating SXC48 improved quality QF indicated by more as well lower pH butyric content. affected content silages. For DF2 silages, at better than chopping, while had good quality, regardless time. The results 16S rRNA sequencing that delayed enhanced bacterial diversity materials changed composition microbes. Kosakonia , Pseudomonas Pantoea jointly dominated fresh material, Lelliottia material. relative abundance from 16.4% control 76.5% 82.0%, respectively. DF also silages . Conclusions Under present research conditions, reduced propionic however, undesirable bacteria, such Lelliottia. effectiveness varied strian both chopping obtained similar benefit. Graphical